Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium.
Identifieur interne : 004214 ( Main/Exploration ); précédent : 004213; suivant : 004215Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium.
Auteurs : Rakesh Minocha [États-Unis] ; Jae Soon Lee ; Stephanie Long ; Pratiksha Bhatnagar ; Subhash C. MinochaSource :
- Tree physiology [ 0829-318X ] ; 2004.
Descripteurs français
- KwdFr :
- Arbres (cytologie), Arbres (génétique), Arbres (physiologie), Azote (métabolisme), Cellules cultivées (MeSH), Milieux de culture (MeSH), Populus (cytologie), Populus (génétique), Populus (physiologie), Putrescine (biosynthèse), Végétaux génétiquement modifiés (cytologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (physiologie).
- MESH :
- biosynthèse : Putrescine.
- cytologie : Arbres, Populus, Végétaux génétiquement modifiés.
- génétique : Arbres, Populus, Végétaux génétiquement modifiés.
- métabolisme : Azote.
- physiologie : Arbres, Populus, Végétaux génétiquement modifiés.
- Cellules cultivées, Milieux de culture.
English descriptors
- KwdEn :
- Cells, Cultured (MeSH), Culture Media (MeSH), Nitrogen (metabolism), Plants, Genetically Modified (cytology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (physiology), Populus (cytology), Populus (genetics), Populus (physiology), Putrescine (biosynthesis), Trees (cytology), Trees (genetics), Trees (physiology).
- MESH :
- chemical , biosynthesis : Putrescine.
- chemical , metabolism : Nitrogen.
- chemical : Culture Media.
- cytology : Plants, Genetically Modified, Populus, Trees.
- genetics : Plants, Genetically Modified, Populus, Trees.
- physiology : Plants, Genetically Modified, Populus, Trees.
- Cells, Cultured.
Abstract
We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.
DOI: 10.1093/treephys/24.5.551
PubMed: 14996659
Affiliations:
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Minocha</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cells, Cultured (MeSH)</term><term>Culture Media (MeSH)</term><term>Nitrogen (metabolism)</term><term>Plants, Genetically Modified (cytology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (physiology)</term><term>Populus (cytology)</term><term>Populus (genetics)</term><term>Populus (physiology)</term><term>Putrescine (biosynthesis)</term><term>Trees (cytology)</term><term>Trees (genetics)</term><term>Trees (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (cytologie)</term><term>Arbres (génétique)</term><term>Arbres (physiologie)</term><term>Azote (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Milieux de culture (MeSH)</term><term>Populus (cytologie)</term><term>Populus (génétique)</term><term>Populus (physiologie)</term><term>Putrescine (biosynthèse)</term><term>Végétaux génétiquement modifiés (cytologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Putrescine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Putrescine</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Arbres</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Arbres</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Milieux de culture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14996659</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>5</Issue><PubDate><Year>2004</Year><Month>May</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium.</ArticleTitle><Pagination><MedlinePgn>551-60</MedlinePgn></Pagination><Abstract><AbstractText>We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Minocha</LastName><ForeName>Rakesh</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>USDA Forest Service, Northeastern Research Station, P.O. 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Minocha</name></noCountry><country name="États-Unis"><region name="New Hampshire"><name sortKey="Minocha, Rakesh" sort="Minocha, Rakesh" uniqKey="Minocha R" first="Rakesh" last="Minocha">Rakesh Minocha</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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